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Molecular characterization of methicillin-resistant Staphylococcus aureus (MRSA) and associated risk factors with the occurrence of goat mastitis.

M. Ijaz

Events

06-24-2020

Abstract:

355
Molecular characterization of methicillin-resistant Staphylococcus aureus (MRSA) and associated risk factors with the occurrence of goat mastitis.
M. Ijaz*, M. Altaf, M. Kashif Iqbal, A. Rehman, M. Avais, A. Ghaffar. University of Veterinary & Animal Sciences Lahore, Punjab, Pakistan.

The current study was designed to determine the prevalence of Staphylococcus aureus (S. aureus) related subclinical mastitis (SCM) and detection of methicillin-resistant Staphylococcus aureus (MRSA) in dairy goats in Pakistan. A total of 385 milk samples were collected from Mianwali (n = 192) and Narowal (n = 193) districts and initially screened for high SCC by using Surf Field Mastitis Test (SFMT). SFMT based positive samples which shows clumping were further processed for the isolation and identification of S. aureus by using mannitol salt agar. The positive samples were subjected to disc diffusion test using oxacillin discs and further confirmed through amplification of mecA gene to detect resistance against Methicillin in S. aureus isolates. Hypothesized risk factors for the occurrence of SCM were recorded and analyzed through logistic regression model in SPSS version 20.0. Initially variables were analyzed by univariable analysis. At significance level of P < 0.2, the variables were tested. Then these variables were included in multivariable logistic regression model. The study revealed 39.2% (151/385) prevalence of SCM by SFMT which was mainly 80.8% (122/151) caused by S. aureus. MRSA prevalence through disc diffusion test was 18.8% (23/122) while PCR based prevalence was 6.5% (8/122). All the study isolates showed 99% homology with MRSA isolates of India, Turkey and Japan with accession numbers MH798869, EU790488 and NG047938 respectively, available in NCBI database. Milker's care and hygienic measures during milking, milk yield, use of teat dips, presence of ticks, mixed type of grazing and services by professionals were key risk factors associated with the occurrence of SCM in goats. This is the first report regarding the molecular characterization of MRSA isolated from dairy goats in Pakistan and the study will be helpful to provide information for developing control strategies against mastitis in goats.

Keywords: subclinical mastitis, dairy goats, methicillin-resistant Staphylococcus aureus.

Biography: Dr. Muhammad Ijaz is working as Associate Professor (Tenured) in the Department of Clinical Medicine and Surgery, University of Veterinary and Animal Sciences, Lahore and holds D.V.M. (UVAS), MPhil (UVAS), Ph.D. (UVAS &USA) & Post. Doc.(Cornell University, USA) degrees in Veterinary Medicine. His published work includes 6 books/booklets and 3 book chapters, 129 published research articles with a total of 151.742 Impact Factor, and 23 oral presentations at International/National professional conferences and 55 published abstracts. He is also reviewer for 10 scientific journals. Dr. Ijaz is also member of editorial board of the Journal of Animal & Plant Sciences.