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Effect of autochthonous Nepalese plants on ruminal methane production in vitro.

E. Vargas-Bello-Pérez


Effect of autochthonous Nepalese plants on ruminal methane production in vitro.
R. Dhakal1, E. Vargas-Bello-Pérez*1, M. González-Ronquillo2, H. H. Hansen1. 1Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen Frederiksberg C, Denmark, 2Facultad de Medicina Veterinaria y Zootecnia, Instituto Literario 100, Universidad Autónoma del Estado de México Toluca, Estado de México, México.

The objective of this study was to determine the use of dried fruits from commonly used Nepalese plants on rumen fermentation in vitro. Two plants and a plant product were tested in 3 concentrations: the fruit of Terminalia chebula (HA), Terminila belliricia (BA) and Triphala Churna (TC), a commercial mixture with 3 equal parts (DM basis) of Phyllanthus emblica, Terminila belliricia, and Terminalia chebula. These plants/product were tested pure and at 20% and 40% of the total sample dry matter in maize silage (MS). Additionally, pure MS was used as a control. These 10 treatments were tested during 2, 48-h fermentations with quadruplicate samples. Rumen fluid was collected from 2 fasting cannulated heifers. Total gas production (TGP: mL at standard temperature and pressure (STP)/g DM), methane production (MP), and volatile fatty acids contents (VFA) were measured or calculated from measurements. Data were analyzed with linear mixed models using R version (3.5.1) with each treatment as a main effect and run as a random variable. Total gas production and MP were less (P < 0.05) than MS (186 mL gas/g DM with 10% methane) for pure samples, but not significantly different from MS for 20 and 40% plants/product mixtures with a reduction in TGP between 5 and 15 and 8 to 16% for methane. Neither total VFA production (mmol/L) nor proportions of propionic acid and acetate (mol/100mol) were significantly different between MS and plant/product and MS mixtures (P > 0.05). The reduction in MP and similarities between total VFA, TGP, and VFA profiles for the plants/product mixtures and pure MS suggest that these can be used with MS at the given inclusion rates. The decrease in methane represents a heat energy value of between 2.3 and 5.5 MJ/kg of DM, which the animal can theoretically use. Therefore, these plants/product may increase productivity while decreasing enteric methane emission.

Keywords: digestibility, fermentation, methane.