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Production of high-purity phospholipid concentrate from buttermilk powder using ethanol-modified supercritical carbon dioxide.

A. Ubeyitogullari

Abstract:

132
Production of high-purity phospholipid concentrate from buttermilk powder using ethanol-modified supercritical carbon dioxide.
A. Ubeyitogullari*, S. S. H. Rizvi. Cornell University Ithaca, NY.

Milk fat globule membrane (MFGM) phospholipids are in considerable demand due to their well-known health benefits and superior functionality to create clean-label products. However, MFGM phospholipid concentrates of commercial utility are still scarce. This study was designed to explore a new strategy using ethanol-modified supercritical carbon dioxide (SC-CO2) to produce MFGM phospholipid concentrate of high purity. The effects of extraction conditions (temperature, pressure, and ethanol concentration) on the yield and phospholipid concentration were investigated. The total lipid and phospholipid contents of commercially available buttermilk powder were determined using the conventional Folch extraction and found to contain 9.0% total lipids, of which 60% was phospholipids. A sequential extraction strategy of using pure SC-CO2 followed by ethanol-modified SC-CO2 was employed to first extract the nonpolar lipids as the first fraction and the phospholipid-rich concentrate was obtained as the second fraction. The total lipid yield of the first fraction was 2.5% which contained only 0.3% phospholipids. On the other hand, the second fraction yielded 4.3% total lipids with a phospholipid content of 76%, which, to the best of our knowledge, is the highest phospholipid concentration obtained from buttermilk powder. The sequential SC-CO2 extraction process achieved a recovery of 97% of the nonpolar lipids and 61% of the phospholipids from buttermilk powder. At the optimized extraction conditions, the major phospholipids in the extract were dihydrosphingomyelin (DHSM; 5%), sphingomyelin (SM; 24%), phosphatidylethanolamine (PE; 22%), phosphatidylserine (PS; 2%), phosphatidylinositol (PI; 3%), and phosphatidylcholine (PC; 44%). The triacylglycerol compositions of the extracts obtained by Folch and SC-CO2 extractions methods were similar. Such phospholipid-rich concentrate offers a new opportunity for value addition to buttermilk powder using a commercially scalable process.

Keywords: buttermilk, phospholipids, supercritical carbon dioxide.