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Simulating the effect of two-day-delayed sealing on the fermentation characteristics and aerobic stability of an alfalfa crop during the early stage of fermentation, treated with a dual strain inoculant.

A. Gallo

Events

06-23-2020

Abstract:

247
Simulating the effect of two-day-delayed sealing on the fermentation characteristics and aerobic stability of an alfalfa crop during the early stage of fermentation, treated with a dual strain inoculant.
A. Gallo*1, F. Ghilardelli1, K. Witt2, I. Eisner2, A. Segura2, G. Copani2. 1Department of Animal Science, Food and Nutrition (DIANA). Universit� Cattolica del Sacro Cuore Piacenza, Italy, 2Chr. Hansen Animal Health & Nutrition H�rsholm, Denmark.

Adverse event during silage making, such as delayed sealing, could influence silage quality and resulting aerobic stability, when fermented for short time. The aims was to study the effects of a silage inoculant on fermentation and aerobic stability (AS) of alfalfa silage evaluated after 14 d of fermentation, in normal ensiling conditions (NORM) or in a challenge situation (DELAY = 2 d delayed sealing and forage sprinkled with water to simulate 7 mm of rainfall). To test this hypothesis, 3 20 l mini-silos/treatment were filled with wilted alfalfa (183 � 4.6 kg DM/m3, 39.6% DM NORM or 35.5% DM DELAY). For both NORM and DELAY, forage was treated with no inoculant (CTR) or with SiloSolve FC (FC) containing 50:50 of Lactobacillus buchneri (DSM22501) and Lactococcus lactis (DSM11037). The target application rate was 150,000 cfu/g of forage. The mini-silos were stored for 14 d at 20�C, meaning the DELAY setting fermented anaerobically for only 12 d. After fermentation, each mini-silo followed an AS test by monitoring the temperature (T) increase inside the mini-silos until they reached +3�C above T ambient. The statistical model examined the fixed effect of ensiling conditions (NORM vs. DELAY), inoculation (CTR vs. FC), and their interaction. The pH increased (4.55 vs. 5.34, P < 0.05) and lactate decreased (3.66 vs. 1.56% DM, P < 0.05) in NORM and DELAY silages, respectively. Use of SiloSolve FC numerically enhanced acetate (2.46 in CTR vs. 3.11% DM in FC, P = 0.16), whereas yeast count was decreased (5.1 vs. 3.9 log10 cfu/g, respectively in CTR and FC; P < 0.05). AS in NORM treatment was increased by 6 d in FC silages compared with CTR (P < 0.05). However, the DELAY reduced AS in both CTR and FC (13 vs. 26 h; P = 0.715). These results indicated that use of SiloSolve FC improves aerobic stability and fermentation even after a very short period of fermentation in the NORM group. In the DELAY, the short fermentation time was insufficient to obtain a stable silage, despite a numerical improvement of FC over CTR.

Keywords: aerobic stability, Lactobacillus buchneri, Lactococcus lactis.