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Isoprostanes reduce production of reactive oxygen species and apoptosis in a bovine model of oxidative stress.

A. K. Putman


Isoprostanes reduce production of reactive oxygen species and apoptosis in a bovine model of oxidative stress.
A. K. Putman*, J. C. Gandy, L. M. Sordillo. Michigan State University College of Veterinary Medicine East Lansing, MI.

Oxidative stress is associated with several economically important diseases in dairy cattle and results in damage to tissue macromolecules. Isoprostanes (IsoP) are molecules generated from interactions between free radicals and membrane phospholipids, thus serving as excellent indicators of free radical-mediated lipid damage during times of oxidative stress. In dairy cattle, IsoP have been detected throughout the lactation cycle, during both health and disease. While IsoP are recognized as excellent biomarkers of oxidative stress, their physiological role remains largely unknown. The vascular endothelium is a primary target of lipid peroxidation during oxidative stress. Thus, this experiment aimed to determine the effect of the most extensively studied IsoP, 15-F2t-IsoP, on bovine endothelial cells during oxidative stress conditions. Bovine aortic endothelial cells (BAEC) were incubated in the presence of 10 nM 15-F2t-IsoP alone and in combination with known oxidizers 2,2′-azobis(2-methylpropionamidine) dihydrochloride (AAPH) and lipopolysaccharide (LPS). 15-F2t-IsoP decreased ROS production in BAEC incubated with AAPH for 12 h compared with cells incubated with AAPH alone. Additionally, 15-F2t-IsoP decreased apoptosis in BAEC incubated with LPS for 12 h when compared with cells incubated with LPS alone. The results of this study indicate that 15-F2t-IsoP may have a cytoprotective role during times of oxidative stress. Future studies should be directed toward investigating if IsoP alter other factors associated with vascular damage during oxidative stress, such as endothelial cell barrier integrity. This research benefits the industry by providing insight into how a well-known biomarker of oxidative stress in dairy cattle may contribute to the pathophysiology of economically important diseases.

Keywords: isoprostane, oxidative stress.

Biography: Ashley graduated from the University of Findlay in 2015 with a B.S. in animal science and biology. She recently completed her DVM at Michigan State University, where she is also a PhD candidate in the Comparative Medicine and Integrative Biology program. Her current research interests include elucidating the physiological role of isoprostanes, a lipid-derived biomarker of oxidative stress, in dairy cattle during health and disease.