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Saccharomyces cerevisiae fermentation products reduce effects of subacute ruminal acidosis on CowPi-predicted functionalities of rumen microbiota in lactating dairy cows.

J. Guo

Events

06-22-2020

Abstract:

148
Saccharomyces cerevisiae fermentation products reduce effects of subacute ruminal acidosis on CowPi-predicted functionalities of rumen microbiota in lactating dairy cows.
J. Guo*1, Z. Zhang3, I. Yoon2, M. Zhou3, L. Guan3, E. Khafipour2, J. Plaizier1. 1University of Manitoba Winnipeg, MB, Canada, 2Diamond V Cedar Rapids, IA, 3University of Alberta Edmonton, AB, Canada.

Cows were randomly assigned to 4 treatments (n = 8) that received a TMR (34.9% NDF, 18.6% starch, DM basis) supplemented with 140 g/d of ground corn (Control), 126 g/d of ground corn and 14 g/d of XPC (XPC, Diamond V Original XPC, Cedar Rapids, IA), 121 g/d of ground corn and 19 g/d of NutriTek (NTL, NutriTek, Diamond V), or 102 g/d of ground corn and 38 g/d of NutriTek (NTH) from 4 wk before until 12 wk after calving. Subacute ruminal acidosis(SARA) challenges were conducted during wk 5 (SARA1) and wk 8 (SARA2) after calving by replacing 20% of the TMR with pellets containing 50% barley and 50% wheat. Rumen samples were collected weekly from wk 3 until wk 10 after calving. DNA was extracted, Illumina sequenced at the V1—V2 regions of the 16S rRNA gene, and analyzed by QIIME2. The OTU counts and sequence tables were constructed in the galaxy implementation of CowPI to predict the Kegg ortholog abundances at the pathway-level. Out of 205 functions, 165 were changed by SARA. The relative abundance of arachidonic acid metabolism increased, but this was reduced by NTH (P = 0.03) during SARA2. The abundance of glycosaminoglycan degradation was lower during NTL than during Control (0.038% vs 0.044%, P = 0.01. The nod-like receptor signaling pathway decreased more during SARA2 than SARA1 (P < 0.01), and NTH increased its relative abundance compared with Control (0.065% vs 0.063%, P = 0.05). Retinol metabolism tended to increase more during SARA2 than SARA1 (P = 0.08), and NTH decreased its abundance compared with Control (0.0031% vs 0.0035%, P = 0.05). Lipopolysaccharide (LPS) biosynthesis and LPS biosynthesis proteins increased during SARA challenges (P < 0.01), which agrees with increases in rumen free LPS observed in a parallel study. SARA challenges altered predicted rumen functionalities related to inflammation, and NutriTek attenuated these changes.

Keywords: Saccharomyces cerevisiae fermentation product, SARA, rumen microbiota.