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Effect of two different approaches of synchronization of follicular wave emergence on follicle turnover, oocyte recovery and quality, and early in vitro developmental competence of embryos in Nili-Ravi buffaloes.

U. Arshad

Events

06-23-2020

Abstract:

240
Effect of two different approaches of synchronization of follicular wave emergence on follicle turnover, oocyte recovery and quality, and early in vitro developmental competence of embryos in Nili-Ravi buffaloes.
M. Sagheer1, F. Ullah1, M. Saleem1, M. Nawaz1, A. Riaz1, U. Arshad*2, N. Ahmad1. 1Department of Theriogenology, Faculty of Veterinary Science, University of Veterinary and Animal Sciences Lahore, Pakistan, 2Department of Animal Sciences, University of Florida Gainesville, FL.

The objective of the present study was to evaluate the effect of 2 methods of follicular wave emergence on follicle turn-over, oocyte recovery (OR), quality of the oocytes (OQ), and early in vitro developmental competence of embryos (IVEP) in Nili-Ravi buffaloes. In a 2 � 2 crossover design, buffaloes (n = 8) were randomly divided to receive either 1) FA [follicular ablation; n = 4], or 2) CIDR-EB [synchronization protocol, n = 4]. In FA group, the ovarian follicles were aspirated (week −1) and subsequently repeated OPU sessions (n = 55) were performed for 7 weeks. However, in CIDR-EB treated buffaloes, a P4 device (CIDR) inserted in the anterior vagina and a single dose of EB (2 mg) and prostaglandin (150 �g) were administered (wk 1). The CIDR was removed on d 7 and repeated OPU sessions (n = 56) were performed. In both groups, a 7-d resting period was provided between each OPU session. Data on the number of follicles, OR, OQ, and IVEP were analyzed using the PROC MIXED and GLIMMIX procedures of SAS. Results (LSM � SEM) revealed that the number of small (3.50 � 0.63 vs.2.69 � 0.60) sized follicles was higher (P < 0.05) in FA- treated, whereas, medium (1.13 � 0.45 vs. 1.59 � 0.45) sized follicles were higher (P < 0.05) in CIDR-EB treated buffaloes. However, the large (0.04 � 0.01 vs. 0.02 � 0.01) sized and total number of follicles (4.65 � 0.88 vs. 4.42 � 0.88) did not differ (P > 0.30) across the treatments. The OR (0.67 � 0.18 vs. 0.53 � 0.20), and OQ (Grade I+II; 0.15 � 0.09 vs. 0.17 � 0.09 and grade III+IV; 0.85 � 0.02 vs. 0.83 � 0.02), remained similar (P > 0.05) in both groups, respectively. Similarly, rates of in vitro maturation (0.66 � 0.18 vs. 0.59 � 0.20), cleavage (0.42 � 0.19 vs. 0.52 � 0.20), and morula (0.13 � 0.13 vs. 0.08 � 0.10) did not differ (P > 0.05) between the groups, respectively. Taken together, CIDR-EB enhances the medium-sized follicles, however, the 2 fundamental approaches of synchronization of wave emergence result in similar efficiency for early IVEP in buffaloes.

Keywords: follicular wave emergence, in vitro developmental competence of embryos, buffalo.