Adsa Logo White Adsa Title White

Blood microRNA profile differs between primiparous and multiparous cows fed a high-concentrate diet.

N. Reisinger




Blood microRNA profile differs between primiparous and multiparous cows fed a high-concentrate diet.
N. Reisinger*1, A. Stauder2, M. Hackl3, J. Faas1, Q. Zebeli2. 1BIOMIN Research Center Tulln, Austria, 2Institute of Animal Nutrition and Functional Plant Compounds, Department for Farm Animals and Veterinary Public Health, Vetmeduni Vienna Vienna, Austria, 3TAmiRNA GmbH Vienna, Austria.

During transition period dairy cows have to deal with several stressful events e.g., change from a fiber-rich close-up diet to a high-concentrate diet. Primiparous cows (PPC) might be more susceptible to these changes than multiparous cows (MPC). The aim of the study was to investigate the blood microRNA (miRNA) profile of PPC and MPC before and during feeding of a high concentrate diet. Eight lactating Simmental cows (73 � 25.4) DIM; 4 PPC, 4 MPC) were used for this study. Cows received a baseline diet (60% roughage; 40% concentrate) followed by a high concentrate diet (HCD) for 4 weeks (40% roughage, 60% concentrate). Blood was collected during baseline diet, wk 1 and wk 4 of HCD. miRNAs were isolated from plasma samples and sequenced using the CleanTag small RNA library prep kit. Sequencing was performed on an Illumina NextSeq 550. Reads were mapped against miRBase using miRDeep2. MiRNA raw read counts were processed using EdgeR and generalized linear models to perform differential expression analysis. P-values were adjusted for multiple testing using Benjamini-Hochberg false discovery rate. Overall, 48 miRNAs were differently expressed in PPC and MPC (P < 0.05). During baseline, 16 miRNAs were differently expressed in PPC cows compared with MPC (P < 0.05). While during wk 1 and 4 of HCD, 26 and 10 miRNAs were differently expressed (P < 0.05), respectively. Differential expression was confirmed for a subset of miRNAs by RT-qPCR. Accordingly, the HCD increased the expression of bta-miRNA-122 by 2.2-fold as well as the expression of bta-miR-30a-5p by 1.6-fold in wk 4 (P < 0.05). These 2 miRNAs were as well affected by parity in wk 1 of the HCD as they were increased in PPC by 3.6-fold and 2.9-fold (P < 0.05), respectively. In addition, bta-miR-192 was increased by 1.5-fold, bta-miR-194 by 1.4-fold and bta-miRNA-100 by 2.0-fold in PPC during wk 1 of the HCD (P < 0.05). Furthermore, bta-miRNA-1246 was increased by 2.1-fold in PPC (P < 0.05). In conclusion, HCD affected miRNAs associated with liver and mammary gland health in both PPC and MPC cows. However, other miRNAs associated with the gut and immune system were differently affected in PPC and MPC. Therefore, PPC might need special attention and might benefit from different management.

Keywords: blood miRNA, parity, high-concentrate diet.