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Variation in bovine colostrum fat content is related to specific lipid species.

R. N. Klopp

Abstract:

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Variation in bovine colostrum fat content is related to specific lipid species.
R. N. Klopp*1, A. Suarez-Trujillo1, C. R. Ferreira2, T. M. Casey1, J. P. Boerman1. 1Department of Animal Sciences, Purdue University West Lafayette, IN, 2Metabolite Profiling Facility, Bindley Bioscience Center, Purdue University West Lafayette, IN.

Colostrum is an essential source of nutrients, energy, and antibodies for neonates. Studies across multiple species associate long-term programming effects of colostrum on health, fertility and production capacity. However, the quality of colostrum varies extensively across cows and herds, including fat content, which is essential for calf temperature regulation and thermogenesis. Understanding what is potentially driving the variation of fat content in colostrum may enable the development of approaches to standardize quality to ensure nutrient needs of calves are met. The objective of this study was to determine if there is a relationship between the percent fat of colostrum and the amount of specific lipids in colostrum. Colostrum was collected from 16 multiparous cows within 2 h of calving and immediately frozen at −20�C until analysis. Colostrum fat percent was measured using the creamatocrit approach, 5.46 � 2.20% (mean � SD) and range from 0.92 to 8.36%. The Bligh and Dyer protocol was used to extract lipids from the colostrum and multiple-reaction monitoring (MRM) profiling was used to measure colostrum lipids on an Agilent 6410 QQQ mass spectrometer (Agilent Technologies). MRM-profiling was divided into 2 phases, a discovery and screening phase. For discovery phase a pooled colostrum sample was profiled, and lipids with intensities ≥1.3-fold in relation to the blank were selected for the screening phase. Individual colostrum samples were profiled for selected lipids. Data were uploaded into MetaboAnalyst 4.0 for statistical analysis following autoscaling for normalization. Correlation analysis, evaluating non-triglycerides, indicated a significant relationship between percent fat and multiple lipids in colostrum, including phosphatidylcholine (PC) 32:1 (P = 0.004, r = 0.67), PC 32:0 (P = 0.02, r = 0.57), PCo 40:0 (P = 0.01, r = −0.62), and PCo 42:4 (P = 0.05, r = −0.50), sphingomyelin (SM) d18:0/24:0 (P = 0.02, r = 0.59), and SM d18:1/24:0 (P = 0.03, r = 0.55). These findings suggest that specific colostrum lipids are related to percent fat, however further studies are required to determine regulatory mechanisms for colostrum fat synthesis.

Keywords: colostrum, fat, lipidome.