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Fermentation parameters and microbiome dynamic of whole-plant corn silage inoculated with Lactobacillus buchneri or in combination with Lactobacillus hilgardii and Pediococcus pentosaceus.

R. Scuderi

Events

06-23-2020

Abstract:

164
Fermentation parameters and microbiome dynamic of whole-plant corn silage inoculated with Lactobacillus buchneri or in combination with Lactobacillus hilgardii and Pediococcus pentosaceus.
P. Drouin1, R. Scuderi*1, E. Apper2. 1Lallemand Specialties Milwaukee, WI, 2Lallemand SAS Blagnac, France.

Aerobic deterioration is a key concern in silage of sugar-rich forage having low buffering capacity, as it can cause low quality or important losses during feed-out. Inoculation of silage with several microbial additives can result not only in limiting the aerobic deterioration but also in reducing losses. The objective of the trial was to evaluate inoculant usage on fermentation losses, fermentation parameters and aerobic stability (AS) of the silage. An ensiling trial (7-L mini-silos) was performed using a conventional hybrid corn silage. The treatments consisted of tap-water (Control), L. buchneri (LB)(Lalsil Fresh HC), and a combination of 2 heterofermentative strains, L. buchneri, L. hilgardii, and the homofermentative P. pentosaceus (Combo)(Lallemand Magniva Platinum 3). Openings were at 30, 60, and 180 d. Fermentation parameters, organic volatile compounds, AS, and nutritional parameters were evaluated. Bacterial composition was also determined using 16S rRNA amplicon sequencing. Design was a randomized complete block, blocked by repetition. One-way ANOVA was performed for each opening. At all 3 openings, lactic acid concentration was similar for Combo and Control silages and lower in LB silage. Lactate/acetate ratio was higher for Combo compared with Control silage. Results obtained with the Combo were indicative of homofermentation. AS was over 240 h by first opening for LB and Combo silages, lower in the control group at 30 and 60 d opening (P < 0.001). 1,2-Propanediol was highest in LB, and lowest in Control silage (P < 0.001). Propionic acid was below detection level. Compared with LB, the Combo significantly decreased losses of fresh matter for all 3 openings (P < 0.001 each openings). Dry matter recovery follows the same trend (P = 0.009). Mean NDF of fresh forage was of 38.8% DM (P = 0.858), but down to 32.5% 30.4% for Control and Combo (P = 0.197). The results will be compared with the bacterial microbiome, which was dominated by Lactobacillaceae. This trial confirmed the use of different bacterial strains improves AS by limiting fermentation losses.

Keywords: microbiome, silage, bacterial additives.