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Effects of probiotics on in vitro digestibility, rumen fermentation, methane and gas production from a corn silage-based TMR.

A. Oyebade




Effects of probiotics on in vitro digestibility, rumen fermentation, methane and gas production from a corn silage-based TMR.
A. Oyebade*, K. G. Arriola, H. Sultana, S. Lee, E. Duvalsaint, F. Amaro, I. Fernandez Marenchino, C. Nino De Guzman, L. Marroquin Pacheco, L. Ghedin Ghizzi, M. Reboucas Pupo, M. Agarussi, L. Ferraretto, A. Adesogan, D. Vyas. University of Florida Gainesville, FL.

The study objective was to evaluate effect of 2 probiotic supplements on in vitro nutrient digestibility, methane (CH4) and volatile fatty acid (VFA) production using in vitro batch fermentation assay. Three ruminally-cannulated cows were used in a 3 � 3 Latin Square design and fed either basal corn silage-based TMR without probiotics (Control; CON), the basal diet top-dressed with mixture of Lactobacillus animalis and Propionibacterium freudenreichii at 3 � 109 cfu/day (BOV) or the basal diet top-dressed with BOV and mixture of Bacillus subtilis and B. licheniformis at 11.8 � 109 cfu/day (BOVC). Each experimental period was 28-d, and rumen fluid was collected from each cow, 3 h after feeding on d 22, 24, 27, and 28 of the experimental period for 4 runs of in vitro batch fermentation assay per period. Rumen fluid collected from each donor cow corresponded to the specific dietary treatment the cow was receiving. Additionally, 2 more treatments (BOV+ and BOVC+) were included in the assay by adding extra dose (1.62 mg/mL) of the 2 probiotic supplements (BOV and BOVC) to the vials containing their respective rumen fluids. A corn silage-based TMR (1mm; 0.5 g) used as substrate was incubated in serum vials with respective rumen fluid buffered with Van soest medium and reducing agent at 39�C. Data were analyzed using GLIMMIX procedure of SAS and differences were considered significant at P ≤ 0.05. Treatments were used as fixed effects while cow and run were considered random factors. No treatment effects were observed on digestibility parameters. The BOV and BOV+ inocula decreased molar proportion of butyrate (13.8 and 13.6%), compared with CON (14.3%), while BOVC+ tended (P = 0.07) to decreased molar proportion of butyrate. BOV+ lowered gas production (143.9 vs 162.8 mL/g DMD), and tended to reduce CH4 production (9.07 vs 11.6 mg/g DMD), compared with CON. BOVC reduced ruminal acetate (50.2 vs 51.1%) and tended to reduce isovalerate (P = 0.07) molar proportion. In conclusion, probiotic supplementation did not change in vitro nutrient digestibility; however, it tended to lower CH4 production.

Keywords: methane, probiotics, rumen fermentation.