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Effect of three different schemes of ovum pick-up on the follicular population, recovery, quality, and in vitro developmental competence of oocytes in Sahiwal cattle.

M. Saleem

Events

06-23-2020

Abstract:

274
Effect of three different schemes of ovum pick-up on the follicular population, recovery, quality, and in vitro developmental competence of oocytes in Sahiwal cattle.
M. Saleem*1, M. Nawaz1, M. Yaseen1, M. Sagheer1, M. R. Yousuf1, A. G. Bajwa2, A. Riaz1. 1Department of Theriogenology, Faculty of Veterinary Science, University of Veterinary and Animal Sciences Lahore, Punjab, Pakistan, 2Department of Microbiology, Faculty of Veterinary Science, University of Veterinary and Animal Sciences Lahore, Punjab, Pakistan.

The objective of the present study was to compare the 3 different schemes of ovum pick-up (OPU) in terms of follicular population, recovery, quality and nuclear maturation of oocytes, and in vitro developmental competence of embryos in Sahiwal cattle. Eighteen (n = 18) Sahiwal cattle (having a normal estrous cycle) were selected and subjected to a 7-d double-dominant follicle puncture (DFP). All the animals were randomly divided into 3 groups following DFP: (1) Twice-weekly (n = 6), (2) Once-weekly (n = 6), and (3) Bi-weekly (n = 6) OPU interval groups. The first OPU was conducted on either 3 to 4 d, 7 d or 14 d after 2nd DFP, depending on the treatment recommended for each group and a total of 60 (n = 60) OPU's were performed for each group. Data on the follicular population, oocytes recovery and total viable oocytes were analyzed by One-way ANOVA and LSD test was used to test the difference between the groups. The data on the oocytes quality, nuclear maturation, cleavage rate and blastocyst rate were analyzed by chi-squared test using SPSS. Results (Mean � SE) and (%) showed that the number of medium-sized follicles (1.16 � 0.21 vs. 0.86 � 0.12 vs. 0.55 � 0.09) and A, B grade oocytes (33.4 vs. 21.5 vs. 21.8%) in the twice-weekly OPU interval group were higher (P < 0.05) compared with once-weekly and bi-weekly OPU interval groups, respectively. The number of oocytes recovered (6.80 � 0.96 vs. 4.80 � 0.42 vs. 4.20 � 0.40) and degenerated oocytes (0.85 � 0.20 vs. 0.43 � 0.12 vs. 0.16 � 0.06) were higher (P < 0.05) in bi-weekly OPU interval group compared with once-weekly and twice-weekly OPU interval group, respectively. Nevertheless, total viable oocytes (2.70 � 0.28 vs. 2.60 � 0.27 vs. 3.30 � 0.46), nuclear maturation (63.6 vs. 51.4 vs. 59.2%), cleavage rate (59.3 vs. 44.2 vs. 56.6) and blastocyst rate (29.0 vs. 18.5 vs. 28.3%) did not differ (P > 0.05) between the groups. In conclusion, twice-weekly OPU interval is a good approach for maximizing Sahiwal cattle's genetic potential within a short period of time without sacrificing the total transferable stage embryos.

Keywords: ovum pick-up, Sahiwal cattle.