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Effects of feeding rumen-protected lysine during the transition period on postpartum uterine health and follicular dynamics of Holstein cows.

A. Guadagnin

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06-23-2020

Abstract:

272
Effects of feeding rumen-protected lysine during the transition period on postpartum uterine health and follicular dynamics of Holstein cows.
A. Guadagnin*1, L. Fehlberg1, B. Thomas1, Y. Sugimoto2, I. Shinzato2, F. Cardoso1. 1University of Illinois, Department of Animal Sciences Urbana, IL, 2Ajinomoto Co. Inc Tokyo, Japan.

Diets fed during the transition period play an important role in the cow's reproductive tract following parturition. We aimed to determine the effects of feeding rumen-protected Lys (RPL, AjiPro-L Generation 3, Ajinomoto Heartland Inc., Chicago, IL) pre- (0.54%DM of TMR) and/or postpartum (0.395%DM of TMR) on uterine health and follicular dynamics of Holstein cows (n = 75). Treatments began at 28d before expected calving and consisted of TMR with RPL pre- and postpartum (LL), with RPL pre- and without RPL postpartum (LC), without RPL pre- and with RPL postpartum (CL), and without RPL pre- and postpartum (CC). Uterine health was evaluated at 4, 7, 10, 13, 15, and 17 DRC by evaluating purulent vaginal discharge (PVD) via Metricheck (MC) Score and MC Smell. Swabs of the endometrium were collected at 15 and 28 DRC. Polymorphonuclear leukocytes (PMN) were evaluated as a percentage of the epithelial cells. Follicular development was monitored via ultrasound every 2 d starting at 7 DRC until 28 DRC. Data were analyzed using MIXED and GLIMMIX procedures in SAS. Cows in C (20.39 � 3.49%) had greater (P < 0.01) proportion of PMN cells in the uterus than cows in L (14.02 � 3.49%); and there was also an effect of POS on PMN, in which cows in LC (18.88 � 3.49%) had greater (P < 0.01) postpartum proportion of PMN cells in the uterus than cows in LL (9.16 � 3.49%). A PRE � POS interaction for PMN was present, as cows in LL (9.16 � 3.49%) had lower (P < 0.01) PMN proportion than cows in LC (18.88 � 3.49%), CL (20.50 � 3.49%), and CC (20.76 � 3.49%). Cows in CL were more likely to develop PVD than cows in LL (95CI = 2.08—61.91, P = 0.01) or LC (95CI = 1.33—23.55, P = 0.02) at 13 DRC. A POS effect (P = 0.02) on the total growth of the first dominant follicle (DF) was detected, in which cows in LC (11.19 � 1.68mm) and CC (11.34 � 1.68mm) had greater total growth of DF than cows in LL (6.32 � 1.68mm) and CL (7.73 � 1.68mm). In conclusion, feeding cows with RPL pre- and postpartum improved uterine health postpartum, and the inclusion of RPL postpartum decreased growth of the first DF.

Keywords: lysine, polymorphonuclear leukocytes, purulent vaginal discharge.