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Effects of carprofen on inflammation biomarkers and health of transition Holstein cows.

S. T. Quanz




Effects of carprofen on inflammation biomarkers and health of transition Holstein cows.
S. T. Quanz*1, A. R. Rodr�guez2, H. A. Bustamante2, L. K. Mamedova1,3, B. J. Bradford1,3. 1Kansas State University Manhattan, KS, 2Universidad Austral de Chile Valdivia, Los Rios Region, Chile, 3Michigan State University East Lansing, MI.

The objective of this study was to analyze the effect of carprofen on transition cow inflammation biomarkers and health. Late-gestation multiparous cows (n = 62) were enrolled at the beginning of the close-up period and randomly assigned to treatments at parturition. Cows received either no treatment (control) or subcutaneous carprofen (1.4 mg/kg BW) 24 — 48 h postpartum. Cows treated with antibiotics or anti-inflammatories during the close-up period were removed from the study (6 treatment, 2 control). Health was observed daily for 21 d postpartum. For the first 120 d of lactation, cows were confined and fed a TMR, and then moved to a rotational grazing system and supplemented. Blood plasma and serum were collected from the coccygeal vein 21 d before expected calving and once weekly for 3 wk postpartum. Serum haptoglobin was determined by enzymatic activity, plasma fibrinogen and total protein concentrations by refractometry, and plasma maresin-1 and adiponectin concentrations by ELISA. Treatment, disease, and time were modeled as fixed effects with parity as a random effect; significance was declared at P < 0.05. Treatment cows had fewer instances of clinical metritis (36% vs. 66%, P ≤ 0.05), but there was no effect on puerperal metritis, displaced abomasum, pneumonia, clinical mastitis, or lameness incidence (P > 0.10). Carprofen had no effect on adiponectin or fibrinogen concentrations (P > 0.10) but increased total plasma protein concentration (P ≤ 0.05) and tended to increase maresin-1 (P = 0.09), a resolving oxylipid. Carprofen tended to decrease plasma haptoglobin (P = 0.07). Cows with clinical metritis had greater concentrations of fibrinogen and haptoglobin (P ≤ 0.05), but there were no disease � treatment interactions (P > 0.10). Carprofen tending to increase adiponectin concentration only among cows with clinical metritis (P = 0.06; disease � treatment interaction P < 0.05). Overall, carprofen decreased the incidence of clinical metritis while also altering biomarkers in a manner suggesting decreased systemic inflammation, consistent with a more successful navigation of the transition period.

Keywords: metritis, maresin-1, adiponectin.