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Effects of non-toxigenic clostridia oral supplementation on production, health, and metabolism of Holstein cows.

F. Cardoso




Effects of non-toxigenic clostridia oral supplementation on production, health, and metabolism of Holstein cows.
F. Cardoso*1, T. Rehberger2, J. Thompson2, A. Smith2, M. Griffin2, F. Cardoso1. 1University of Illinois Urbana, IL, 2Arm & Hammer Animal and Food Production Waukesha, WI.

There is scarce evidence on the effects of clostridia contaminated TMR on the performance of dairy cows. Forty-one multiparous Holstein cows DIM (192 � 111) were randomly assigned to 1 of 4 dietary treatments. The treatments were based on the clostridia load supplementation. Cows received the treatments by top-dress and oral bolus. Treatments were: CON (no added clostridia), LOW (669 � 515 cfu/g of TMR), MED (2953 � 2272 cfu/g of TMR) and HIG (7179 � 5151 cfu/g of TMR). Cows were balanced with regard to parity, body condition score, and previous lactation milk yield. Observations for each variable during the adaptation phase (phase 1; cows consumed a basal diet without receiving treatment for one week) were used as a covariate for the corresponding treatment and statistical inference phase (phase 2; wk 1 through 9). A subset of cows (n = 15) were rumen-cannulated and used for rumen papillae (RP) biopsy on the last day of wk 9. Milk and dry matter intake (DMI) were obtained daily. Fecal pH was measured every 2 weeks. Cows in LOW tended to have increased (P = 0.07) DMI than cows in MED (24.9 and 22.5 kg/d, respectively). There was no difference (P = 0.18) in DMI as a percentage of BW among treatments. There was no treatment effect for milk yield (P = 0.78). Cows in CON had higher (P = 0.05) fecal pH than cows in LOW and MED (6.67vs6.57 and 6.54, respectively). Cows in CON had greater (P = 0.01) RP gene expression of toll like receptor 2 (TLR2) than cows in LOW, MED and HIG (2.02vs 0.93, 0.74, 1.1, respectively); the expression of the gene 3-hydroxy-3-methylglutaryl-CoA synthase 2 (HMGCS2) was greater (P = 0.02) for CON and HIG compared with LOW and MED (1.44, 1.11vs 0.93; 0.74, respectively) and there was a tendency (P = 0.08) for the RP gene expression of cluster of differentiation 14 (CD14) to be greater for MED than HIG (1.16vs 0.66, respectively). In conclusion, cows in MED tended to have decreased DMI compared with cows in LOW. Additionally, based on RP gene expression, cows in CON had improved RP integrity than cows challenged with clostridia

Keywords: Clostridium, TMR, gene expression.