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Preparation and evaluation of a film-forming teat dip containing chitosan for the prevention of mastitis.

H. Zhang



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Preparation and evaluation of a film-forming teat dip containing chitosan for the prevention of mastitis.
H. Zhang*, H. Jiang, Z. Han, Y. Mao, Z. Yang. College of Animal Science and Technology, Yangzhou University Yangzhou, Jiangsu, PR China.

Postmilking teat dip is an effective procedure for prevention of mastitis in dairy cows during lactation. Film-forming teat dip was developed to improve the effectiveness of postmilking disinfection by leaving a germicidal film on the teats to against mastitis pathogens. Chitosan is a nontoxic and biodegradable polysaccharide that has received attention due to its great film-forming capacity and antibacterial effect. Aim of the trial was to evaluate the efficacy of a film-forming teat dip containing chitosan in preventing mastitis of dairy cows. In vitro antibacterial test, the film-forming teat dip agent (FFTDA), with 1.0% 50 kDa chitosan and 4% povidone iodine, showed better antibacterial effect against Escherichia coli, Staphylococcus aureus, Streptococcus agalactiae, Bacillus cereus than the agent with no chitosan (P < 0.05). Then the efficacy of FFTDA was compared with a conventional iodine postmilking teat dip on 48 healthy Chinese Holstein dairy cows. The right mammary gland half was assigned to receive FFTDA (treated group), the other side was dipped into conventional iodine bath (control group). Treatments were applied for 4 weeks, for 3 milkings per day. Milk samples were collected from each quarter at 0, 2, 4 weeks and analyzed for nutrients composition, somatic cell count (SCC) and somatic cell score (SCS). Subclinical mastitis was diagnosed by LMT test every week. After clinical trial of 4 weeks, there were no significant differences in milk fat content, protein content, lactose content, total solids content, SCC and SCS between treated group and control group. In the end of the trial, the incidence of subclinical mastitis teat of treated group and control group were 9.38% and14.58%, respectively. SCS was significantly lower than the beginning of the trial in the treated group (P < 0.05), while there was no significant difference in the control group. The results indicated that this film-forming teat dip containing chitosan can effectively reduce SCS, and it has a good effect on the control of subclinical mastitis in dairy cow.

Keywords: bovine mastitis, chitosan, film-forming teat dip.