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Comparison of ruminal and serum short-chain fatty acids concentrations in dairy cows with different levels of milk production.

L. C. Caixeta

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06-23-2020

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Abstract:

T60
Comparison of ruminal and serum short-chain fatty acids concentrations in dairy cows with different levels of milk production.
L. C. Caixeta*, B. O. Omontese, C. Chen, Y. Guo. University of Minnesota Saint Paul, MN.

Short-chain fatty acids (SCFA) are the product of microbial fermentation in the rumen and are the main source of energy used by dairy cows for milk production. This pilot study aimed to compare the concentration of SCFA in ruminal fluids and serum collected from second lactation Holstein dairy cows with different levels of milk production. Rumen and serum samples were collected at 65 � 8.5 DIM from 24 randomly selected cows. Cows were divided in 3 phenotypic groups based milk production: low producing (LP; n = 8) with 41.8 � 1.2 kg/d yield, moderate producing (MP; n = 10) with 54.9 � 1.1 kg/d yield, and high producing (HP; n = 6) with 61.2 � 1.5 kg/d yield. Rumen samples were collected using non-invasive oro-ruminal sampling device (Flora Rumen Scoop, Profs-Product) and serum samples were collected by puncture of the coccygeal blood vessels using vacuum tubes (Vacutainer; Becton Dickinson, Franklin Lakes, NJ). Rumen and serum samples stored at −80�C until metabolomics analysis. The concentrations of acetic, propionic, and butyric acids in rumen fluid and serum were determined using liquid chromatography-mass spectrometry. The differences of SCFA concentrations in rumen fluid and serum between the cohorts was analyzed using 2-tailed t-test with Bonferroni-Holm correction for multiple comparisons. The concentration of SCFA in rumen fluid did not differ between phenotypic groups; however, the concentration of propionic acid in serum was lower in the LP (135 � 40 ug/g) cows when compared with that in the MP (235 � 132 ug/g) and HP groups (185 � 55 ug/g) (LP vs. MP, P = 0.04; Lp vs HP, P = 0.09). Our results suggest that the concentration of propionic acid in serum is associated with milk production more than the concentration of propionic acid in rumen fluid.

Keywords: short-chain fatty acids, milk production, rumen fluid.