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Feeding frequency effect on behavioral patterns and bacterial community composition in Holstein and Jersey cows.

V. M. De La Guardia

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06-24-2020

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Abstract:

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Feeding frequency effect on behavioral patterns and bacterial community composition in Holstein and Jersey cows.
V. M. De La Guardia*, H. A. Paz. Animal and Dairy Sciences Department, Mississippi State University, $Starkville, MS .

Multiple factors can influence the composition of the gastrointestinal microbiome of dairy cattle. This study aimed to evaluate the effect of feeding frequency on cow behavior and on the bacterial community composition of the rumen and hindgut. A 28-d trial was conducted with 10 Holstein and 10 Jersey cows fed for ad libitumconsumption a diet comprised of 70% forage and 30% concentrate using the Calan Broadbent Feeding System. Within breed, cows were randomly assigned to treatments: 1 (06:00 h) or 2 (06:00 and 18:00 h) feed deliveries per day. Behavioral patterns were monitored on d 23 and 24 with data recorded at intervals of 15 min and classified into one of the following activities: lying, standing, ruminating, eating, drinking, or milking. On d 28, 5 concomitant collections of rumen and fecal samples were performed at intervals of 6 h via esophageal tubing and fecal grab, respectively. The bacteriome composition of these samples was determined through sequencing of the V4 region of the 16S rRNA gene using the Illumina MiSeq platform. Raw sequences were processed using the QIIME2 pipeline and PERMANOVA was performed using R (v3.5.1). Behavioral data were analyzed using PROC MIXED of SAS (9.4) Behavioral patterns were not affected by feeding frequency within breed (P ≥ 0.15); however, both ruminating (6.7 vs. 5.5 � 0.2 h) and laying (15.3 vs. 13.4 � 0.6 h) times were greater in Holstein than Jersey cows (P = 0.04). Shannon diversity was higher for Holstein compared with Jersey cows (P = 0.01) and for feces compared with rumen samples (P < 0.01). Overall bacterial community composition differed by breed (P = 0.01) and sample site (P < 0.01) but not by feeding frequency (P = 0.95) or time (P = 0.52). These results indicate that breed and gastrointestinal site are factors that drive the overall bacterial community composition while feeding frequency and diurnal cycle are not.

Keywords: cattle behavior, feeding frequency, gastrointestinal microbiome.

Biography: My name in Vanessa De La Guardia, I'm from a small country in latin america called Panama and I've work with dairy cattle since my childhood. I got my undergraduate degree in Honduras where I majored in Agricultural Production. Currently I'm a master student at Mississippi State University in the department of Animal and Dairy Sciences, where I'm doing research on microbiome dynamics of dairy cattle.