Adsa Logo White Adsa Title White

Bacteria count differences in used bedding and bulk tank milk based on bedding type in five New York dairy farms.

V. Alanis


Bacteria count differences in used bedding and bulk tank milk based on bedding type in five New York dairy farms.
V. Alanis*1, M. Zurakowski2, D. Pawloski2, D. Nydam1, P. Ospina1. 1Cornell University Ithaca, NY, 2Cornell University Cobleskill, NY.

The objectives were to evaluate the differences in bacteria counts between bulk tank milk and 5 different used bedding types (manure solids, paper fiber, straw, recycled sand, and sand) from lactating pens from 5 New York Dairies. Sampling occurred monthly over one year (July 2018—2019). Bedding was collected making sure to avoid manure, from 3 to 5 representative stalls following a strict standard operating procedure to minimize sampling variability, and a bulk tank milk sample was collected on the same day. Bedding (cfu/g) and bulk tank milk (cfu/mL) were cultured for coliform, non-coliforms, and Streptococcus spp. counts. In milk, Staphylococcus spp. counts were also reported. The Kruskal-Wallis test was used to evaluate bacterial count differences in individual bedding and milk samples. The only bacteria count difference in milk was Staphylococcus spp. where farm bedding with paper fiber had the highest counts and sand had the lowest (P = 0.01). There were no differences in other bacteria counts in milk (P > 0.1). Although there were significant statistical differences between bedding type with organic bedding having slightly higher bacteria counts, overall, organic bedding showed similar bacterial counts than inorganic bedding (Table 1). However, it is important to note that farms using organic bedding were re-bedding more frequently than those using sand.Table 1.

Bacteria group countUsed bedding type
Manure solidsn=44Paper Fibern=70Strawn=18Recycled sandn=60Sandn=45
Bedding samples1
 Coliforms6.7�0.9 (6.4—6.9)a5.9�0.7 (5.8—6.1)b6.7�0.6 (6.4—6.9)a4.9�0.7 (4.8—5.1)c4.9�0.7 (4.7—5.2)c
 Non-coliforms(n)28.1�0.4 (7.8—8.2)a6.5�1.0 (6.2—6.5)b(7)7.1�0.6 (6.8—7.3)b5.8�0.7 (5.6—5.8)c(6)5.9�0.6 (5.7—6.1)c(10)
Streptococcus spp.9.1�0.3 (8.9—9.2)a9.2�0.5 (9.0—9.3)a9.5�0.5 (9.2—9.7)b8.3�0.6 (8.1—8.4)c8.5�0.9 (8.2—8.8)d
Bedding frequencyEvery dayTwice weeklyEvery dayWeeklyWeekly
Bulk tank samples3
 Staphylococcus spp.2.3�0.6 (1.8-2.8)AB3.3�0.3 (2.9-3.6)A2.7�0.6 (2.0-3.4)AB2.6�0.4 (2.2-2.9)AB2.2�0.4 (1.9-2.5)B
a—dMeans with different lowercase superscripts within a row differ (P < 0.0001).A,BMeans with different uppercase superscripts within a row differ (P < 0.01).1Mean � SD, (95% CI) bacteria counts (log10 cfu/g) in bedding over a year.2Where (n) indicates number of samples removed due to no growth culture result.3Mean � SD, (95% CI) bacteria counts (log10 cfu/mL) in bulk tank over a year.

Keywords: bedding, bacterial counts, milk quality.

Biography: I graduated as a veterinarian in 2009 from UNAM in M�xico. I worked as a clinical assistant for 30 private dairy farms for 2 years. Then I was part of an initiative for the Mexican Government to aid a group of 15 indigenous dairy cow farms. During 3 years, I oversaw udder health and milking practices on a 500-cow dairy farm. Then I decided to continue my studies to pursue a Master's in the Program for Animal Production and Health, specifically in the milk quality area. Now I am working on my Ph.D. in Animal Science at Cornell University.