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Effect of inoculant dose and time of ensiling on the fermentation and aerobic stability of corn silage.

X. Liu

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06-23-2020

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Abstract:

T50
Effect of inoculant dose and time of ensiling on the fermentation and aerobic stability of corn silage.
X. Liu*, C. Mellinger, J. Stypinski, N. Moyer, A. Colberg. Dairy Nutrition and Silage Fermentation Laboratory, University of Delaware Newark, DE.

We evaluated the effect of an inoculant on the fermentation and aerobic stability of corn silage. Fresh whole-plant corn (Master's Choice 6150) was harvested (19-mm chop length) from 2 separate fields (on different days, 42.25 and 37.68% DM, respectively) with a pull behind chopper equipped with a kernel processor (roll gap setting 1.5 mm). Forage from each field was untreated (CTRL) or treated with a low level of SilageProB (SP) (SP-L; American Farm Products, Saline, MI; application rate: 200,000 cfu of /g of L. buchneri (LB)/ g of fresh weight and 50,000 cfu of Pediococcus pentosaceus, 50,000 cfu of P. acidilactici and isolated enzymes from Aspergillus oryzae and Bacillus subtilis or, a high level of SP (SP-H; differing from SP-L in that the application rate for LB was 400,000 cfu/g). Treatments were applied as individual replicates in quintuplicate and ensiled in 7.5L bucket silos (density of 240 kg of DM/m3) at 21? for 30 and 90 d. Data were analyzed by ANOVA as a completely randomized design in a factorial arrangement of treatments (2 d of ensiling � 3 inoculation treatments � 2 fields) using JMP with significance declared at P < 0.05. At 30 d the concentration of acetic was higher only for SP-H compared with CTRL, but it was higher for SP-L and SP-H compared with CTRL at 90 d. Both levels of inoculant increased concentrations of 1,2 propanediol at both ensiling times. Inoculation increased the numbers of lactic acid bacteria compared with CTRL. There was a day x treatment interaction for aerobic stability and yeasts. At 30 d, only SP-H resulted in fewer yeasts and improved stability when compared with CTRL. However, at 90 d, both levels of inoculant markedly decreased the numbers of yeasts (inoculated = <log 1 vs. CTRL = 4.85 log cfu/g) and improved aerobic stability (inoculated average of 225 h) compared with CTRL (44 h). These data show that when using the strain of LB in SP, a higher application rate was needed for a small improvement in aerobic stability after 30 d of ensiling, but that either level of inoculation was markedly efficacious when silage fermented for 90 d.

Keywords: corn silage, microbial inoculant, aerobic stability.