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Effect of inoculant dose and time of ensiling on the fermentation and aerobic stability of snaplage.

C. A. Mellinger

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06-22-2020

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Abstract:

M125
Effect of inoculant dose and time of ensiling on the fermentation and aerobic stability of snaplage.
C. A. Mellinger*, X. J. Liu, J. D. Stypinski, N. A. Moyer, L. Kung Jr.. University of Delaware Newark, DE.

We evaluated the effect of a microbial inoculant on the fermentation and aerobic stability of snaplage. Snaplage was harvested (19-mm chop length) in 2 years (different hybrids and different farms) at 62% and 63% DM, respectively. For each year, snaplage was untreated (CTRL) or treated with a low level of SilageProB (SP) (SP-L; American Farm Products, Saline, MI; application rate: 200,000 cfu of /g of L. buchneri (LB)/ g of fresh weight and 50,000 cfu of Pediococcus pentosaceus, 50,000 cfu of P. acidilactici and isolated enzymes from Aspergillus oryzae and Bacillus subtilis) or a high level of SP (SP-H; differing from SP-L in that the application rate for LB was 400,000 cfu/g).Treatments were applied as individual replicates in quintuplicate and ensiled in 7.5L bucket silos (density of 240 kg of DM/m3) at 21? for 30 and 90 d. Data were analyzed by ANOVA as a completely randomized design in a factorial arrangement of treatments (2 d of ensiling � 3 inoculation levels � 2 years) using JMP with significance declared at P < 0.05. Inoculation had no effect of numbers of lactic acid bacteria at 30 d but it was increased by both levels after 90 d. Inoculation did not affect the concentration of lactic acid or ethanol. There was a 3-way interaction on the concentration of acetic acid. Inoculation had no effect on the concentration of acetic acid at 30 d but at 90 d, SP-H increased acetic acid compared with CTRL, but only in 1 of the 2 yr. Inoculation numerically, but not statistically, decreased numbers of yeasts in silage (CTRL = 4.42, SP-L = 4.35, SP-H = 4.06 log cfu/g fresh weight). There was a day � inoculation effect on aerobic stability as there was no difference in stability of silages at 30 d (67, 70, and 74 h for CTRL, SP-L, and SP-H, respectively). However, at 90 d stability was higher for SP-H (102 h) than CTRL (69 h) but it was similar to SP-L (82 h). These data show that for the strain of LB used in SP, improvements in aerobic stability were not observed after 30 d and a high dose of LB was required to show efficacy at 90 d.

Keywords: snaplage, microbial inoculant, aerobic stability.