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Adipose and muscle transcriptome analysis of dairy calves supplemented with 5-hydroxytryptophan.

S. Field



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Adipose and muscle transcriptome analysis of dairy calves supplemented with 5-hydroxytryptophan.
S. Field*, M. Marrero, F. Pe�garicano, J. Laporta. Department of Animal Sciences, University of Florida Gainesville, FL.

Serotonin is a monoamine that regulates processes such as energy balance and immune function. Manipulating this pathway in growing dairy calves could promote growth and development by modulating functions and signaling pathways within key organs. We characterized the adipose and muscle transcriptome of pre-weaned calves with increased serotonin bioavailability through the elucidation of differentially expressed genes (DEGs) and biological pathways. Holstein bull calves (21 � 2 d old) were fed milk replacer (8 L/d) supplemented with saline (CON, 8 mL/d n = 4) or 5-hydroxytryptophan (5-HTP, 90 mg/d, n = 4) for 10 d. Calves were euthanized on d 10 to harvest adipose and muscle tissue for RNA-Sequencing and histological analyses. RNA-Seq (NovaSeq 6000) data analysis included read quality control and processing (FastQC and Trim Galore), genome alignment (Hisat and ARS-UCD1.2), transcript reconstruction (Cufflinks and Cuffmerge) and read counting (htseq-count). Differentially expressed genes (FDR ≤ 0.10) were detected using the R package edgeR. The enrichment of Gene Ontology terms with DEGs was analyzed using Fisher's exact test. To quantify cell number and surface area, H&E staining of adipose and muscle was performed. 5-HTP-fed calves had greater number (P = 0.001) but smaller adipocytes (P = 0.08) and similar muscle fiber number relative to CON. Overall, there were 9 and 18 DEGs within adipose and muscle, respectively. Notably, interferon δ inducible protein 47 (IFI47) was upregulated in both tissues (log2 fold change = 6.2 and 6.8). Significant biological pathways (P < 0.01) related to cell processes, such as regulation of cell proliferation, apoptosis and differentiation were identified in muscle. Other significant pathways were related to inflammatory response, neutrophil chemotaxis and cytokine-mediated signaling. In adipose, pathways related to intracellular signaling of small GTPases, extracellular matrix remodeling and cell migration were identified (P < 0.05). Manipulating the serotonin pathway in pre-weaned calves through 5-HTP supplementation influences biological functions and signaling pathways which play essential roles in metabolic homeostasis and immune function.

Keywords: serotonin.

Biography: Sena Field is a first year Ph.D. student at the University of Florida working with Dr. Jimena Laporta. Her research focus is on serotonin and it's impact in dairy calves and cows on the cellular and molecular level with both�in vivo�and�in vitro�models.