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Effects of Lactobacillus hilgardii 4785 and L. buchneri 40788 on the bacterial community of high-moisture corn.

E. Benjamim da Silva



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Effects of Lactobacillus hilgardii 4785 and L. buchneri 40788 on the bacterial community of high-moisture corn.
E. Benjamim da Silva*1, R. M. Savage1, M. L. Smith1, S. A. Polukis1, P. Drouin2, L. Kung Jr.1. 1University of Delaware Newark, DE, 2Lallemand Specialties Inc Milwaukee, WI.

We evaluated the effect of 2 additives (Lallemand Animal Nutrition, Milwaukee, WI) on the bacterial community dynamics of high moisture corn (HMC) during ensiling. The HMC (71% DM) was untreated (CTR), treated with L. buchneri NCIMB 40788 (LB; 600,000 cfu/g of fresh wt.), treated with L. hilgardii CNCM I-4785 (LH; 600,000 cfu/g), or treated with LB + LH (LBLH; 300,000 cfu of each/g). Three individually replicated lab silos (7.5 L) for each treatment were packed (669kg DM/m3) and ensiled for 10, 30, and 92 d between 21 and 23�C before being sampled for the analysis of the bacterial community by the sequencing of the 16S rRNA V3-V5 region using Illumina MiSeq. Relative abundance (RA) data were analyzed as a 4 � 3 factorial design with the fixed effects of additive, length of ensiling, and their interaction using JMP (SAS Institute Inc.). The distance matrix was calculated using Phyloseq (McMurdie and Holmes, 2013). The untreated grains before ensiling formed a cluster separated from ensiled samples and CTR silages formed clusters separated from treated silages. No separation among LB, LH, and LBLH was observed but treated HMC ensiled for 92 d clustered apart from treated HMC ensiled for 10 and 30 d. The LB, LH, and LBLH HMC had similar RAs of Lactobacillaceae, Enterobacteriaceae, Leuconostocaceae, and Streptococcaceae. Treated HMC had a higher (P < 0.05) Lactobacillaceae RA than CTR (71.20 vs. 19.09%) at all lengths of storage and CTR had a higher (P < 0.05) Enterobacteriaceae RA than treated HMC (23.72 vs. 4.37%). At 10 and 30 d, CTR had a higher (P < 0.05) RA of Leuconostocaceae (58.46 vs. 16.20%) and Streptococcaceae (4.39 vs. 1.51%) than treated HMC, but at 90 d the RA of those families were similar among treatments (32.22 and 0.68%, respectively). Lactobacillaceae dominance on treated HMC explained their higher aerobically stability compared with CTR, previously observed by Polukis et al. (2016). The additives increased the RA of Lactobacillaceae and reduced the RA of other families, such as Enterobacteriaceae, which comprises strains that can reduce the nutritive value and hygienic-sanitary quality of HMC.

Keywords: bacterial community, Lactobacillus hilgardii, silage.