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Evaluating different doses of probiotics on rumen fermentation, nutrient digestibility, and methane production using batch fermentation assay.

L. P. Marroquin

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06-22-2020

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Abstract:

M129
Evaluating different doses of probiotics on rumen fermentation, nutrient digestibility, and methane production using batch fermentation assay.
L. P. Marroquin*, K. G. Arriola, P. Tian, F. X. Amaro, C. A. Nino de Guzman, I. Fernandez, P. Schmidt, A. Oyebade, H. Sultana, S. Lee, E. J. C. Duvalsaint, M. M. C. N. Agarussi, D. Vyas. University of Florida Gainesville, FL.

The aim of this study was to evaluate the effect of different doses of probiotic on in vitro rumen fermentation, nutrient digestibility and methane emissions using TMR or corn silage as substrates. Two probiotics (A: mixture of Lactobacillus animalis and Propionibacterium freudenreichii; and B: mixture of Lactobacillus animalis, Propionibacterium freudenreichii, Bacillus subtilis and B. licheniformis) were applied at doses 0 (Control), 1.63 (Low), 3.25 (Medium), and 4.87 mg/mL (High) with substrate (TMR or corn silage; 0.5 g; 1 mm) in triplicates for each run for a total of 3 runs in experiment. Rumen fluid was collected and pooled from 3 ruminally-cannulated lactating dairy cows 2 h after morning feeding. Buffered rumen fluid (52 mL) along with substrate and probiotics were incubated for 24 h at 39�C. Gas production was measured at 0, 6, 9, 12 and 24 h and methane concentration was measured after 24 h of incubation. Data were analyzed using PROC GLIMMIX of SAS. Dose of each probiotic was used as fixed effect while run was used as random factor in the model. Linear and quadratic contrasts were tested, and significance was declared at P < 0.05, while tendency was declared at 0.05 < P ≤ 0.10. No effects were observed with Probiotic A on dry matter digestibility (DMD), gas production or methane concentration, compared with Control. However, NDF digestibility (NDFD) tended to increase in corn silage with Probiotic A, compared with Control (30.9 vs. 24.6%; P = 0.06) while no effects were observed with TMR. No effects were observed with Probiotic B on DMD, gas production or methane concentration, regardless of substrate used; however, NDFD tended to increase in TMR, compared with Control (56.5 vs. 55.3%; P = 0.05) while no effect was observed with corn silage. In conclusion, regardless of the dose used, probiotics tended to improve NDFD; however, responses were substrate specific.

Keywords: corn silage, in vitro fermentation, probiotic.