Supplementation with sunflower or soybean oil: Ruminal fluid fatty acid profile in a Rusitec system.
C. Cajarville
Events
06-22-2020
Abstract:
M133
Supplementation with sunflower or soybean oil: Ruminal fluid fatty acid profile in a Rusitec system.
G. Casarotto1, A. Britos1, S. Carro2, I. Vieitez3, C. Dauber3, C. Cajarville*1. 1Departamento de Producci�n Animal (IPAV), Facultad de Veterinaria, UdelaR San Jos�, Uruguay, 2Departamento de Ciencia y Tecnolog�a de los Alimentos, Facultad de Veterinaria, UdelaR Montevideo, Uruguay, 3Departamento de Ciencia y Tecnolog�a de Alimentos (CYTAL), Facultad de Qu�mica, UdelaR Montevideo, Uruguay.
The aim was to evaluate the effect of including 2 levels of sunflower or soybean oil to a forage-based diet on the rumen fluid fatty acid (FA) profile. In a Rusitec system 5 diets were evaluated: C (control, 65% alfalfa hay and 35% concentrate; 16% CP, 33% NDF, 3.4% EE), SF6 (C + sunflower oil added until reaching 6% EE of diet DM), SF7.5 (C + sunflower oil added until reaching 7.5% EE of diet DM), SB6 (C + soybean oil added until reaching 6% EE of diet DM) and SB7.5 (C + soybean oil added until reaching 7.5% EE of diet DM), incubating 8g of DM in Nylon bags. Inoculum was obtained from 2 rumen fistulated cows fed with the C diet and McDougall artificial saliva was infused at 3%/h. Three runs of 10 d (5-d adaptation period plus 5 d of sampling) were performed. Samples were taken from fermentation units at the same time as the bags were changed. Ruminal fluid FA profile was determined by GC. Data were analyzed according to a repeated measures model and means separated by LSMEANS. Sunflower and soybean oils inclusion resulted in a decrease of C16:0 (P = 0.003) and increased levels of C18:1 t11 (trans-vaccenic acid, TVA; P < 0.001). Diets SF7.5, SB6 and SB7.5 decreased the concentration of saturated FA with respect to control (P = 0.041; Table 1). Results indicated that adding sunflower or soybean oils up to 7.5% EE of total DM in the diet, did not increase C18:2 c9 t11 (CLA) levels, but increased its precursor TVA and may decrease saturation process.Table 1. Rumen fluid FA profile (g/100 g of FA)
Fatty acid | C | SF6 | SF7.5 | SB6 | SB7.5 | SEM | P-value | ||
T | D | T � D | |||||||
C16:0 | 12.12a | 9.75b | 9.10b | 9.08b | 9.25b | 0.826 | 0.003 | 0.643 | 0.990 |
C18:0 | 3.75 | 3.51 | 2.87 | 2.58 | 3.14 | 0.666 | 0.261 | 0.908 | 0.669 |
C18:1 t11 | 0.51b | 0.75a | 0.75a | 0.73a | 0.75a | 0.040 | <.001 | 0.545 | 0.877 |
C18:1 c9 | 40.69 | 43.05 | 44.42 | 44.97 | 44.55 | 1.794 | 0.067 | 0.773 | 0.713 |
C18:2 c9,c12 | 30.07 | 30.94 | 28.87 | 28.20 | 28.80 | 0.846 | 0.125 | 0.181 | 0.981 |
C18:3n-3 | 0.48 | 0.36 | 0.24 | 0.32 | 0.21 | 0.093 | 0.268 | 0.572 | 0.786 |
C18:2 c9,t11 | 0.41 | 0.12 | 0.17 | 0.33 | 0.19 | 0.107 | 0.357 | 0.475 | 0.730 |
SFA | 20.21a | 18.85ab | 17.66b | 17.77b | 18.31b | 1.166 | 0.041 | 0.689 | 0.579 |
MUFA | 40.74 | 43.13 | 44.48 | 45.01 | 44.61 | 1.824 | 0.067 | 0.775 | 0.718 |
PUFA | 30.55 | 31.30 | 29.11 | 28.51 | 29.01 | 0.885 | 0.112 | 0.214 | 0.988 |
Keywords: rumen fermentation, lipids, fatty acid.