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Supplementation with sunflower or soybean oil: Ruminal fluid fatty acid profile in a Rusitec system.

C. Cajarville

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06-22-2020

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Abstract:

M133
Supplementation with sunflower or soybean oil: Ruminal fluid fatty acid profile in a Rusitec system.
G. Casarotto1, A. Britos1, S. Carro2, I. Vieitez3, C. Dauber3, C. Cajarville*1. 1Departamento de Producci�n Animal (IPAV), Facultad de Veterinaria, UdelaR San Jos�, Uruguay, 2Departamento de Ciencia y Tecnolog�a de los Alimentos, Facultad de Veterinaria, UdelaR Montevideo, Uruguay, 3Departamento de Ciencia y Tecnolog�a de Alimentos (CYTAL), Facultad de Qu�mica, UdelaR Montevideo, Uruguay.

The aim was to evaluate the effect of including 2 levels of sunflower or soybean oil to a forage-based diet on the rumen fluid fatty acid (FA) profile. In a Rusitec system 5 diets were evaluated: C (control, 65% alfalfa hay and 35% concentrate; 16% CP, 33% NDF, 3.4% EE), SF6 (C + sunflower oil added until reaching 6% EE of diet DM), SF7.5 (C + sunflower oil added until reaching 7.5% EE of diet DM), SB6 (C + soybean oil added until reaching 6% EE of diet DM) and SB7.5 (C + soybean oil added until reaching 7.5% EE of diet DM), incubating 8g of DM in Nylon bags. Inoculum was obtained from 2 rumen fistulated cows fed with the C diet and McDougall artificial saliva was infused at 3%/h. Three runs of 10 d (5-d adaptation period plus 5 d of sampling) were performed. Samples were taken from fermentation units at the same time as the bags were changed. Ruminal fluid FA profile was determined by GC. Data were analyzed according to a repeated measures model and means separated by LSMEANS. Sunflower and soybean oils inclusion resulted in a decrease of C16:0 (P = 0.003) and increased levels of C18:1 t11 (trans-vaccenic acid, TVA; P < 0.001). Diets SF7.5, SB6 and SB7.5 decreased the concentration of saturated FA with respect to control (P = 0.041; Table 1). Results indicated that adding sunflower or soybean oils up to 7.5% EE of total DM in the diet, did not increase C18:2 c9 t11 (CLA) levels, but increased its precursor TVA and may decrease saturation process.Table 1. Rumen fluid FA profile (g/100 g of FA)

Fatty acidCSF6SF7.5SB6SB7.5SEMP-value
TDT � D
C16:012.12a9.75b9.10b9.08b9.25b0.8260.0030.6430.990
C18:03.753.512.872.583.140.6660.2610.9080.669
C18:1 t110.51b0.75a0.75a0.73a0.75a0.040<.0010.5450.877
C18:1 c940.6943.0544.4244.9744.551.7940.0670.7730.713
C18:2 c9,c1230.0730.9428.8728.2028.800.8460.1250.1810.981
C18:3n-30.480.360.240.320.210.0930.2680.5720.786
C18:2 c9,t110.410.120.170.330.190.1070.3570.4750.730
SFA20.21a18.85ab17.66b17.77b18.31b1.1660.0410.6890.579
MUFA40.7443.1344.4845.0144.611.8240.0670.7750.718
PUFA30.5531.3029.1128.5129.010.8850.1120.2140.988

Keywords: rumen fermentation, lipids, fatty acid.