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Evaluating the correlation of digital dermatitis infection and genetic variants to predict gene resistance in Holstein cattle.

G. M. Canny

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06-24-2020

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Abstract:

W23
Evaluating the correlation of digital dermatitis infection and genetic variants to predict gene resistance in Holstein cattle.
G. M. Canny*1, C. L. Hendley1, W. B. Smith1, B. W. Jones1,2, J. N. Waddell1. 1Tarleton State University Stephenville, TX, 2Texas A&M AgriLife Research Stephenville, TX.

Digital dermatitis (DD) is a contagious bacterial hoof infection. Determining genetic resistance of DD will allow dairy producers to select for this trait, therefore reducing lameness and treatment cost. This study evaluated the correlation of infection and genetic variants to predict genetic resistance of DD resistance in Holstein cattle on a commercial dairy farm in central Texas. Lactating dairy cattle were scored on an M0 (early lesions) to M4 (chronic lesions) scale. Blood samples were collected on lactating cows (n = 45) from the coccygeal vein for genomic DNA isolation. Cows were identified as having a lesion (n = 35) and not having a lesion (n = 10). Four previously reported SNPs were tested on genomic DNA to determine correlation to DD resistance by qPCR melt curve analysis. SNPs in the 3′UTR of C94T, A22DG, interleukin-8 (IL8) and exon 3 of toll-like receptor 4 (TLR4) were analyzed by Chi-Square in SAS (Version 9.4 SAS Institute, Inc., Cary, NC) to determine the frequencies of different genotypes present among the resistant and affected animals. One SNP in Bta-IL8 resulted in a significant chi-square analysis (P = 0.03). These results indicate a genetic correlation to resistance to DD within the Bta-IL8 gene. This qPCR melt curve analysis can lead to a fast, affordable genotyping assay to select for cows genetically resistant to this costly infection.

Keywords: digital dermatitis, gene resistance, interleukin-8.