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Evaluation of an enzymatic lignocellulolytic complex on corn silage fermentation profile.

B. C. Agustinho



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Evaluation of an enzymatic lignocellulolytic complex on corn silage fermentation profile.
B. C. Agustinho*1,2, L. M. Zeoula1, L. F. Ferraretto2, H. F. Monteiro2, M. R. Pupo3, L. G. Ghizzi4, M. C. N. Agarussi2, C. Heinzen Junior2, J. R. Vinyard2, S. L. Bennett2, A. P. Faciola2. 1Universidade Estadual de Maring� Maring�, Paran�, Brazil, 2University of Florida Gainesville, FL, 3Escola Superior de Agricultura Piracicaba, S�o Paulo, Brazil, 4Universidade de S�o Paulo Pirassununga, S�o Paulo, Brazil.

Lignin, present in plants cell wall, reduces cellulose and hemicellulose degradation by ruminal microorganisms. However, some organisms produce enzymes capable of degrading lignin and cellulose that can modify the chemical composition, and consequently, the silage fermentation profile. Therefore, the objective of this study was to evaluate the effects of the addition of an enzymatic lignocellulolytic complex over time on fermentation profile in corn silage. The enzymatic lignocellulolytic complex consisted of laccase, Mn peroxidase, lignin peroxidase, and cellulase and it was produced in the lab, under controlled conditions and the enzymatic concentration and activities were determined. Experimental design consisted of 30 treatments (5 enzymatic levels � 6 opening time points), with 4 mini-silos per treatment. The levels tested were 0, 8.7, 17.4, 26.1, and 34.8 mg enzyme/kg of fresh matter. Enzymatic complex was added and manually mixed in the chopped whole plant corn before ensiling. Samples were ensiled in vacuum-sealed bags and opened at 0, 1, 2, 3, 7, and 30 d after ensiling. The samples were used to make the extract to determine pH, NH3-N, and organic acids. Data were analyzed as a completely randomized design in a 5 � 6 factorial arrangement of treatments using the GLIMMIX procedure in SAS. There was no interaction (P > 0.05) between enzymatic levels and opening time points for the parameters evaluated. Also, there was no difference (P > 0.05) in total acids concentration in the silage among the tested enzymatic levels, as well as in the molar proportion of the main acids (lactic, propionic, acetic, and butyric acids), consequently, no difference was observed in the pH. The NH3-N concentration was also not affected (P > 0.05) by the enzymes. However, the pH decreased and the total acids and NH3-N concentration increased over time (P < 0.01). Our results indicate that the addition of this enzymatic lignocellulolytic complex during ensiling did not affect corn silage fermentation profile.

Keywords: fiber, fibrolytic enzymes, lignin.