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Detecting the bacterial variation of recycled manure solids for use as bedding in freestalls.

H. Wu



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Detecting the bacterial variation of recycled manure solids for use as bedding in freestalls.
H. Wu*, N. Zheng, J. Wang. Laboratory of Quality and Safety Risk Assessment for Dairy Products of Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences Beijing, China.

Recycled manure solids (RMS) was separated from cow feces as bedding. However, the influence of bedding microflora on milk bacteria remains unclear. To investigate the variation of bacteria in samples of RMS process and compare the contribution to microbe in mastitis /healthy bedding to milk microbe. Three consecutive days in 2019, the samples of rectum feces from healthy /mastitis dairy cows were collected. All feces were mixed and then subjected to fecal mixing pool, extrusion, stove, sun-cure according to the RMS process. Samples were collected for each step. Meanwhile, the Mastitis/healthy cowshed bedding and mastitis/tank milk were collected in the farm of Hebei Province. All samples were used to investigate the presence of pathogenic bacteria of mastitis by qPCR mastitis screening kit. And the bacterial diversity of those sample was determined by the variable region of v3-v4 in the flora determined by MiSeq. The Qiime2 software, SPSS, Galaxy and R software were used to analyze the Miseq data. All pathogenic bacteria of mastitis were not found in all samples except the milk samples of mastitis. The microflora similarity of faces and bedding was low in the mastitis/healthy sheds. Moreover, Lefse results showed that bacterial proportion of feces from healthy dairy cows was significant different with those from mastitis cows. F16 (1.8 � 1.3%), Erysipelototrichaceae (1.4 � 1.4%) and Coriobacteriaceae (0.2 � 0.2%) had a higher proportion in the feces of healthy dairy cows, while only Streptococcaceae (0.5 � 0.1%) had higher proportion of mastitis feces (P < 0.05). The enrichment of microorganisms in rectal feces decreased during the RMS processed steps. Compared with rectal feces, there were a higher proportion of Xanthomadaceae (9.2 � 2.2%), Sphingobacteriaceae (2.6 � 1.4%), Saprospiraceae (4.4 � 2.4%), Phyllobacteriaceae (6.7 � 2.4%), Flammeovirgaceae (1.0 � 0.6%), Flavobacteriaceae (9.1 � 4.9%) and Alteromonadaceae (3.7 � 2.1%) in the bedding samples. The predominant bacteria in milk was not abundant in feces or bedding materials. In general, RMS for use as bedding may not increase the incidence of mastitis in dairy cows.

Keywords: recycled manure solids, microflora.