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Effect of Pogostemon cablin extract on proliferation and differentiation of 3T3-L1 preadipocytes and improve insulin resistance.

C. Xu

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06-24-2020

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Abstract:

W69
Effect of Pogostemon cablin extract on proliferation and differentiation of 3T3-L1 preadipocytes and improve insulin resistance.
H. Y. Gao, C. Xu*, Y. Z. Cui, R. X. Chang. Heilongjiang Bayi Agricultural University Daqing, Heilongjiang, China.

To study the effect of Pogostemon cablin on the promotion of 3T3-L1 preadipocytes differentiation and the regulation of glucose and lipid metabolism in adipocytes. Different concentrations of Pogostemon cablin (0.5, 1.0, 2.5 and 5.0 mg/mL) were added into cell culture plate on the first day, On the eighth day, lipid droplets accumulated in cytoplasm of the differentiated preadipocytes were observed by red O staining and quantified by colorimetry. ELISA method was used to detect intracellular triglyceride (TG)levels. Western blot was used to determine PPARγ2 protein expression. Tetramethylazazole blue (MTT) method was used to analyze the effect of pogostemon cablin on the proliferation and differentiation of 3T3-L1 cells. Glucose oxidase-peroxidase method was used to detect glucose concentration in mature adipocytes.The results show that Pogostemon cablin can inhibit the proliferation of 3T3-L1 preadipocytes and promote the differentiation of cells to mature adipocytes (P < 0.05).With the increase of pogostemon cablin concentration, gradually increased lipid storage, gradually increased TG level (P < 0.05).Compared with the induced differentiation group, the expression of PPARγ2 protein in pogostemon cablin group (0.5, 1.0, 2.5 and 5.0 mg/mL)was significantly increased (P < 0.05).Compared with the insulin-resistant mature 3T3-L1 adipocyte group, it was found that pogostemon cablin can significantly promote glucose consumption and improve insulin resistance in mature adipocytes (P < 0.01).Pogostemon cablin can promote the differentiation of 3T3-L1 preadipocytes, promote the accumulation of intracellular lipid droplets and regulate the metabolism of glucose and lipids, thereby improving insulin resistance.

Keywords: 3T3-L1 preadipocytes, differentiation, insulin resistance.