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Effects of ground flaxseed on ruminal microbiome composition in Jersey cows during the grazing season.

D. C. Reyes

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06-24-2020

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Abstract:

W88
Effects of ground flaxseed on ruminal microbiome composition in Jersey cows during the grazing season.
D. C. Reyes*1, E. A. Latham2, L. H. P. Silva1, B. J. Isenberg1, A. F. Brito1. 1University of New Hampshire Durham, NH, 2Bezoar Laboratories Bryan, TX.

Ground flaxseed (GFLX) has been used as a supplement to increase the energy density of dairy diets and the concentration of polyunsaturated fatty acids in milk fat, which have multiple benefits for human health. However, the impact of GFLX on the ruminal microbiota profile of grazing dairy cows remains poorly understood. Therefore, the effects of supplementing cool-season herbage with 10% of diet DM as GFLX on the ruminal microbiota composition of organic certified Jersey were examined in the present study. Ten lactating Jersey cows (112 � 49 DIM) were blocked in pairs by milk production or DIM and, within pair, randomly assigned to control (CON; soybean meal and ground corn grain mix as 10% of total DMI) or GFLX (10% of total DMI) in a randomized complete block design experiment with repeated measures over time. Each experimental period lasted 28 d (n = 4). Ruminal fluid samples were collected after the morning milking (~0700 h) for 2 consecutive days using a stomach tube. Microbial diversity of ruminal samples was determined using the 16s rRNA gene (V1-V3 region) and the Illumina MiSeq sequencing platform. Sequencing data were processed and analyzed with QIIME software. The statistical model included the fixed effects of treatment, period, block, their interactions, and the random effects of cow nested in treatment and was run in SAS with repeated measures. The most abundant phyla (Firmicutes and Bacteroidetes; 49.2 and 40.7% relative abundance (RA), respectively; P > 0.05), families (Sphingobacteriaceae and Clostridiacea; 11.9 and 10.0% RA, respectively; P > 0.05), and genera (Mucilaginibacter and Rikenella; 9.3 and 8.8% RA, respectively; P > 0.05) were unaffected by GFLX. However, GFLX increased (P < 0.05) the RA (%) of the bacterial phylum Lentisphaerae (0.002 vs. 0.0007 � 0.001) and tended (P = 0.08) to increase those of Tenericutes (2.22 vs. 1.47 � 0.22) compared with the CON. At a 10% DMI inclusion level, GFLX did not impact rumen microbiota of grazing Jersey cows.

Keywords: organic, microbiome, pasture.