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Characterization of pregnancy associated glycoproteins (PAG) and progesterone (P4) as a predictor of twins and conceptus loss in high-risk pregnancy Holstein cows.

P. M. Peixoto

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06-23-2020

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Abstract:

T95
Characterization of pregnancy associated glycoproteins (PAG) and progesterone (P4) as a predictor of twins and conceptus loss in high-risk pregnancy Holstein cows.
P. M. Peixoto*1, A. M. Hubner1, L. L. Cunha1,2, W. M. Coelho Jr1, K. G. Pohler3, N. W. Dias4, V. R. G. Mercadante4, I. F. Canisso1,2, F. S. Lima1,5. 1Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois Urbana, IL, 2Department of Comparative Biosciences, College of Veterinary Medicine, University of Illinois Urbana, IL, 3Department of Animal Science, Texas A&M University College Station, TX, 4Department of Animal and Poultry Sciences, Virginia Tech University Blacksburg, VA, 5Department of Population Health and Reproduction, University of California Davis, CA.

The objective of this study was characterizing plasma concentration of PAG and P4 as predictor of twins and conceptus loss in high-risk pregnancy Holstein cows. High risk pregnancy (HR) were characterized using transrectal ultrasonography 37 d post AI based on the following criteria: small embryo size (SE, embryo <15 mm, n = 10), slow heartbeat (SH, < 60 beats per minute, n = 11), extra amniotic membrane (EM, additional amniotic membrane, n = 3). A cohort of twins (TW, n = 41) diagnosed at d 37 post AI was also enrolled. Each HR and TW cow was paired with a same parity cow carrying a normal singleton at d 37 post AI (CON, n = 65). Blood samples were collected to measure PAG and P4 at 37, 44, and 51 post AI. Statistical analysis was performed using ANOVA, logistic regression and receiver operation characteristics (ROC) with JMP. Pregnancy loss at d 51 post AI was greater (P < 0.01) in HR than CON and TW (CON = 1.5%; HR = 87.5%; TW = 12.2%). Concentration of PAG at d 37 post AI did not differ (P = 0.75) among groups (CON = 5.3 � 0.7; HR = 4.8 � 1.2; TW = 4.0 � 0.9 ng/mL). Concentration of P4 at d 37 post AI was greater in TW than HR and CON, and lower (P < 0.01) in HR than CON cows (CON = 7.0 � 0.3; HR = 5.9 � 0.4; TW = 8.4 � 0.3 ng/mL). Regression and ROC analysis for PAG at d 37 post AI did not find a threshold to predict pregnancy loss (P = 0.24) or twins (P = 0.30). Regression and ROC analysis for P4 at d 37 post AI found that a threshold of 6.5 ng/mL predicted (P < 0.01) pregnancy loss with an area under the curve (AUC) of 0.65, and threshold of 7.2 ng/mL predicted (P < 0.01) twins with AUC of 0.70. In summary, pregnancy loss and twins were predicted with only moderate accuracy by P4 concentration at d 37 post AI and PAG concentration at d 37 post AI were not able to generate a threshold to predict pregnancy loss and twins in Holstein lactating cows

Keywords: pregnancy loss, twins, progesterone.

Biography: Awarded DVM degree in 2015 by Universidade Federal de Goias, Brazil. Participation on experiments on Cornell at 2014 during mandatory internship. Research Assistant at university of Illinois during 2015-2016. Performed private practice in Brazil, focused on dairy cattle health and reproduction. Initiate the Master;s program at the Unviersity of Illinois in 2019 and will be presenting my defense during spring 2020. My areas of major interest are bovine theriogenology, physiology and immunology.