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Microbial composition of fecal transplant inoculum from dairy calf feces.

G. S. Slanzon

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06-23-2020

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Abstract:

T22
Microbial composition of fecal transplant inoculum from dairy calf feces.
G. S. Slanzon*, L. M. Parrish, S. C. Trombetta, W. M. Sischo, C. S. McConnel. Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Washington State University Pullman, WA.

GI disease is the most common illness in pre-weaned dairy calves and can lead to dysbiosis, an imbalance of the microbial composition of the gut. Therefore, restoring the microbial composition of the gut is paramount to overcoming the effects of dysbiosis. Fecal microbial transplant (FMT) therapy offers the potential for restoring the microbiota balance by transferring fecal matter from a healthy donor into a diseased recipient. In this study, consistently healthy calves of different breeds 5—24 d of age with fecal scores ≤ 2 out of 4 were used as fecal donors to create the FMT inoculum. A total of 358 frozen fecal samples from 73 calves were combined to prepare a single FMT slurry. All fecal samples tested negative for Salmonella and were processed under aerobic conditions. Samples (~130g each) were combined in a commercial blender with 650 mL of 0.85% saline. Fecal material was sequentially sieved (mesh size 35 and 60) and the slurry was centrifuged (15 min at 6,000 rpm). Supernatant was removed and the gelatinous organic material above the pellet was vacuumed. The slurry was re-suspended in a 1:1.25 saline-10% glycerol solution. Composite feces was allocated into size 00 capsules (Capsuline enteric coated) which were placed within size 0 Capsuline gelatin capsules and frozen at −80�C before administration. Three samples (1g) of the final FMT product were sequenced for the V3-V4 region of the 16S rRNA gene. The average microbial composition of the FMT product samples at the phylum level included 56.9% Actinobacteria, 38.1% Firmicutes, 3.4% Bacteroidetes, and 1.1% Proteobacteria. At the genus level, the product consisted of 47.3% Bifidobacterium, 11.1% Blautia, 9.7% Lactobacillus, 8.5% Collinsella and 2.1% Fecalibacterium. The predominant species were Bifidobacterium longum (21.5%), Bifidobacterium pseudolongum (15.3%), Collinsella aerofaciens (8.5%) and Fecalibacterium prausnitzii (2.2%). Alpha diversity was quantified by the total number of observed species. The FMT product had an average of 200 observed species with an abundance of microorganisms associated with gut health (Bifidobacterium, Fecalibacterium).

Keywords: fecal transplant, dairy, calves.

Biography: PhD student at College of Veterinary Medicine, Washington State University.�MSc in Animal Science at University of Sao Paulo/ESALQ, Brazil. My research is focused on dairy calf health and wellbeing.