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Effects of feeding rumen-protected lysine during the transition period on postpartum immunometabolic gene expression and morphology of uterine samples of Holstein cows.

A. Guadagnin

Abstract:

M21
Effects of feeding rumen-protected lysine during the transition period on postpartum immunometabolic gene expression and morphology of uterine samples of Holstein cows.
A. Guadagnin*1, L. Fehlberg1, B. Thomas1, Y. Suginomoto2, I. Shinzato2, F. Cardoso1. 1University of Illinois, Department of Animal Sciences Urbana, IL, 2Ajinomoto Co. Inc Tokyo, Japan.

Methionine has an impact on the reproductive immune function and metabolism of dairy cows. However, whether lysine plays a similar role is unknown. We aimed to determine the effects of feeding rumen-protected Lys (RPL, AjiPro-L Generation 3, Ajinomoto Heartland Inc., Chicago, IL) pre- (PRE, 0.54%DM of TMR) and/or postpartum (POS, 0.395%DM of TMR) on mRNA gene expression profiles of endometrial samples of Holstein cows. Seventy-three (n = 73) multiparous Holstein cows were randomly assigned to 1 of 4 dietary treatments in a randomized, complete block design with a crossover of a diet with RPL (L) or without (C). Treatments consisted of TMR with RPL pre- and postpartum (LL), with RPL pre- and without RPL postpartum (LC), without RPL pre- and with RPL postpartum (CL), and without RPL pre- and postpartum (CC). Endometrial tissue samples were collected through uterine biopsy at 28 d relative to calving and analyzed for expression of genes related to inflammation and protein metabolism, and stained with hematoxylin and eosin for morphology evaluation. Data collected were analyzed using the MIXED procedure in SAS. There was a tendency for an effect of POS (P = 0.06) on the gene expression of mucin 1 mRNA (MUC1) and mucin 4 mRNA (MUC4), in which cows in L postpartum had greater expression of MUC1 (1.15 � 0.48) and MUC4 (1.05 � 0.43) than cows in C (0.83 � 0.48 and 0.22 � 0.43, respectively). A PRE x POS interaction was observed for the expression of interleukin-10 mRNA (IL10), where cows in LL (11.31 � 1.40) had greater (P < 0.01) expression of IL10 than cows in CC (4.57 � 1.40), CL (4.00 � 1.40), or LC (6.06 � 1.40). There was no difference in the number of endometrial glands (P = 0.20) nor on the number of cells per gland (P = 0.14). In conclusion, feeding RPL postpartum impacts uterine immune response, through increasing the expression of mRNAs that encode glycoproteins involved in the protection and renewal of epithelial cells, and also increasing anti-inflammatory cytokine IL10 mRNA expression.

Keywords: gene expression, lysine, mucin.