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Effects of an immunomodulatory feed additive on biomarkers of inflammation and oxylipid profile in blood of transition cows.

C. S. Takiya



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Effects of an immunomodulatory feed additive on biomarkers of inflammation and oxylipid profile in blood of transition cows.
C. S. Takiya*1, L. K. Mamedova1,2, L. Sordillo2, J. Gandy2, M. Garcia3, E. E. Gultepe4, D. E. Nuzback3, B. J. Bradford1,2. 1Kansas State University Manhattan, KS, 2Michigan State University East Lansing, MI, 3Phibro Animal Health Teaneck, NJ, 4Afyon Kocatepe University Afyonkarahisar, Turkey.

Dairy cows experience an inflammatory state during the postpartum period even in the absence of disease. However, cows are prone to developing metabolic diseases when inflammation is exacerbated. Oxylipids are signaling molecules derived from oxidation of long-chain fatty acids that exert complex control over inflammation. OmniGen-AF (OMN, Phibro Animal Health, Teaneck, NJ) is a commercial feed additive that has shown positive impacts on immunity and performance of dairy cows. Twenty-eight multiparous cows were blocked by expected calving date and assigned to control or OMN (56 g/d top-dressed) from the day of dry-off (−67 � 3.0 d relative to actual calving date) until 49 d in milk. Blood samples were collected at enrollment, d −30, −14, −7, 1, 14, 28, and 42 relative to parturition (RTP) for assessment of plasma haptoglobin (Hp) and α-1-acid glycoprotein (AGP) concentrations; samples from d −14, 1, 7, 14 RTP were analyzed for oxylipid profiles. Haptoglobin and AGP were analyzed based on peroxidase activity and by ELISA (ICL Inc., Portland, OR), respectively. Plasma oxylipids were analyzed by LC/MS/MS. Rectal temperature was measured daily after parturition. Data were submitted to ANOVA with fixed effect of treatment, time, their interaction, and random effects of cow and block; fixed effects of parity group, temperature and humidity-index, and all interactions were also tested. Significance was declared at P < 0.05. Cows fed OMN had similar (P = 0.11) rectal temperature as CON (38.67 vs. 38.72 � 0.033�C, respectively). No treatment nor treatment � time effects were detected for Hp or AGP. Among 33 oxylipids detected, OMN increased (P = 0.018) plasma 6-keto prostaglandin-1α concentration, which is a stable metabolite of prostacyclin I2. Treatment � time interactions (P ≤ 0.048) were observed for thromboxane B2 and 12-hydroxyheptadecatrienoic acid (derived from arachidonic acid via cyclooxygenase), where OMN increased blood concentrations of these metabolites on d −14 RTP. This study demonstrated that OMN can alter pro-inflammatory and resolving class oxylipids.

Keywords: eicosanoid, immunity, rectal temperature.