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Effects of a flavonoid-rich extract on plasma sirtuin 1 and metabolic biomarkers in mid-lactation Holstein cows.

S. T. Quanz



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Effects of a flavonoid-rich extract on plasma sirtuin 1 and metabolic biomarkers in mid-lactation Holstein cows.
S. T. Quanz*1, L. K. Mamedova1,2, D. E. Schimek3, S. E. Schuling3, B. J. Bradford1,2. 1Kansas State University Manhattan, KS, 2Michigan State University East Lansing, MI, 3NutriQuest Mason City, IA.

Sirtuins have recently been shown to influence the antioxidant regulation of several cellular functions. Sirtuin1 (Sirt1), an intracellular deacetylase enzyme, is central in protecting the cell from reactive oxygen species. The aim of this study was to assess the effect of a commercially available flavonoid-rich extract (FRE) in mid-lactation cows. Mid-lactation Holstein cows (n = 31) producing ≥40.8 kg milk/d were blocked by parity, days in milk, and milk production, then randomly assigned to treatment within block. Treatment cows received 10 g/d of FRE (Relieve, NutriQuest, Mason City, IA) administered in a pellet distributed by an automated milking system at each milking, whereas control received a nutritionally equivalent pellet without the FRE. Cows were housed in a freestall barn equipped with a robotic milking system which they entered free-choice throughout the day. Blood plasma was collected from the coccygeal vein before treatment and on d 30 and 60 of the study. Statistical models evaluated fixed effects of covariate data, treatment, parity group (1, 2, or 3+), block, and time, with cow as a random effect; significance was declared at P < 0.05. There were no overall treatment effects on plasma concentrations of free fatty acids (FFA), advanced oxidation protein products (AOPP), Sirt1, trolox equivalent antioxidant capacity (TEAC) or β-hydroxybutyrate (BHB; all P > 0.10). Across treatments, Sirt1, TEAC, and FFA increased over time (d 60 vs. d 30) while AOPP decreased. Parity 3+ cows had greater Sirt1 concentrations than parity 1 and tended (P = 0.07) to be greater than parity 2. There were parity � time interactions for Sirt1 and FFA. Parity 1 cows had a greater increase in Sirt1 over time compared with parity 2 cows. Likewise, FFA concentrations increased to a greater extent for parity 2 cows vs. parity 1 and parity 3+ cohorts. No significant associations were found between concentrations of Sirt1 and other measured biomarkers in plasma. In conclusion, FRE supplementation did not alter plasma metabolite concentrations in mid-lactation cows that were likely not under oxidative stress.

Keywords: metabolites, sirtuin, oxidative stress.