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Comparison of rumen fermentation and microbiome of rumen samples collected via cannula or stomach tube in lactating dairy cows.

K. G. Arriola

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06-24-2020

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Abstract:

W100
Comparison of rumen fermentation and microbiome of rumen samples collected via cannula or stomach tube in lactating dairy cows.
N. Pathak, H. Guan, K. G. Arriola*, C. Nino de Guzman, I. Fernandez, F. X. Amaro, A. Oyebade, L. F. Ferraretto, D. Vyas. University of Florida Gainesville, FL.

The objective of this study was to compare fermentation profile, and microbial diversity from rumen samples collected using rumen cannula or stomach tube in lactating dairy cows. Three ruminally cannulated lactating dairy cows were used as rumen fluid donors in a 3 � 3 Latin Square design. The experimental period was 28 d and rumen fluid was collected 2 h after feeding on d 21 and d 23 of each experimental period. Treatments were rumen fluid collected from Stomach tube (ST) or from rumen cannula (C). Both samples were collected within 10 min from same animal on same day. The pH of rumen contents was measured immediately after fluid collection and rumen samples were analyzed for volatile fatty acid (VFA), ammonia-N (NH3-N) concentration, and microbiome analysis. Data were analyzed using GLIMMIX procedure of SAS. Treatments and period were used as fixed factor while cow was used as a random factor. Significance was declared at P ≤ 0.05. Rumen pH was greater for ST compared with C (6.87 vs 6.25; P < 0.01). However, NH3-N (15.2 vs 10.6 mg/dL; P < 0.01) and total VFA (121.8 vs 95.5 mM; P < 0.01) was greater for C compared with ST. The rumen fluid collection methods had no effects on molar proportion of acetate (P = 0.21) and propionate (P = 0.16); however, acetate-to-propionate ratio tended to increase with ST compared with C (P = 0.08). The majority of sequences obtained with microbiome analysis belonged to Bacterioidetes, Firmicutes, and Cyanobacteria, regardless of the rumen fluid collection method. However, the abundance of Bacteriodetes was greater (P < 0.01) while abundance of Firmicutes was lower (P < 0.01) for ST compared with C. No effects were observed on the abundance of phylum Cyanobacteria (P = 0.13). The rumen fluid collection methods had no effects on Chao 1 (P = 0.14) and Shannon index (P = 0.21). In conclusion, molar proportion of individual VFA, Chao1, and Shannon index were similar while rumen pH, NH3-N, and total VFA were affected by the rumen fluid collection method.

Keywords: microbiome, rumen fluid, stomach tube.